Evaluation of Protamine Deficiency and DNA Fragmentation in Two Globozoospermia Patients Undergoing ICSI
نویسندگان
چکیده
The aim of this report has been evaluating protamine content and DNA integrity of two patients with globozoospermia undergoing Intra Cytoplasmic Sperm Injection (ICSI). Semen analysis was carried out according to WHO criteria. Protamine deficiency and DNA fragmentation was assessed using Chromomycin A3 and sperm chromatin dispersion assay respectively. ICSI and chemical activation were carried out on inseminated oocyte. Both cases demonstrated high degrees of protamine deficiency, while one of the cases indicated high level of DNA fragmentation, too. High fertilization rates were achieved in both cases. However, embryo transfer did not lead to implantation or pregnancy. Artificial oocyte activation overcomes low fertilization rate reported in cases with high level of protamine deficiency. In the present study, failed implantation in one of the cases may be caused by high DNA fragmentation.
منابع مشابه
Evaluation of Protamine Deficiency and DNA Fragmentation in Two Globozoospermia Patients Undergoing ICSI
The aim of this report has been evaluating protamine content and DNA integrity of two patients with globozoospermia undergoing Intra Cytoplasmic Sperm Injection (ICSI). Semen analysis was carried out according to WHO criteria. Protamine deficiency and DNA fragmentation was assessed using Chromomycin A3 and sperm chromatin dispersion assay respectively. ICSI and chemical activation were carried ...
متن کاملP-48: Evaluation of Sperm ChromatinStructure in Patients with Partial Globozoospermia
Background: Globozoospermia is an uncommon disorder of sperm morphology associated with severe male infertility. Patients who contain both normal and round-headed sperm are called partial globozoospermic. It is possible that round-headed sperm may have an abnormal chromatin structure and /or DNA strand breaks. Moreover, sperm DNA fragmentation and protamine deficiency is shown to be associated ...
متن کاملDifferentiation Between the Effect of Protamine Deficiency and Failed Oocyte Activation on Fertilization Post ICSI
Sperm premature chromosomal condensaion (PCC) and failed oocyte activation are considered to result in failed fertilization post intracytoplasmic sperm injection (ICSI). Recent studies also suggested that sperm protamine deficiency may induce PCC. The ain of this study was to assess the effect of failed oocyte activation and protamine deficiency on failed ferilization post ICSI and to indicate ...
متن کاملP-13: Evaluation of Sperm Acrosin Activity and Chromatin Structure in Varicocele Patients Before and after Varicocelectomy
Background: Evaluation and comparison of total acrosin activity along with DNA integrity in the semen samples obtained from the varicocele individuals before and after the surgery and also in fertile individuals. Materials and Methods: Semen parameters, protamine deficiency, DNA fragmentation and Total acrosin activity were measured in patients with a clinical diagnosed varicocele of grade II o...
متن کاملP-81: Evaluation of DNA Fragmentation in Patients with Total Globozoospermia
Background: Infertility is estimated to affect up to 15% of couples of reproductive age, and male factors contribute to 50% of these cases. Globozoospermia syndrome is a severe form of teratozoospermia, with an incidence of less than 0.1%. It is characterized by round sperm head. The most prominent feature of globozoospermia is the malformation of the acrosome, which is totally absent in the to...
متن کامل